LIQUID-CHROMATOGRAPHIC DETERMINATION OF ACRIFLAVINE AND PROFLAVINE RESIDUES IN CHANNEL CATFISH MUSCLE

A liquid chromatographic (LC) method was developed for determination o f acriflavine (ACR) and proflavine (PRO) residues in channel catfish m uscle. Residues were extracted with acidified methanol solution, and e xtracts were cleaned up with C-18 solid-phase extraction columns. Resi due concentrations were determined on an LC cyano column, with spectro photometric detection at 454 nm. Catfish muscle was individually forti fied with ACR (purified from commercial product) and PRO at concentrat ions of 5, 10, 20, 40, and 80 ppb (5 replicates per level). Mean recov eries from fortified muscle at each level ranged from 86 to 95%, with relative standard deviations (RSDs) of 2.5 to 5.7%. The method was app lied to incurred residues of ACR and PRO in muscle after waterborne ex posure of channel catfish to commercial acriflavine (10 ppm total dye for 4 h). RSDs for incurred residues of ACR and PRO were in the same r ange as those for fortified muscle. Low residue concentrations (<1% of exposure water concentration) suggested poor absorption of ACR and PR O in catfish.