Defense reaction of pine seeds: distribution of protein bodies extract from Pinus nigra seeds in agar medium plates

Diffusion and distribution of protein bodies (PB) extract from Pinus nigra seeds in agar medium plates was studied. After application of PB extract on filter paper discs it was placed on agar plates for 20 days. Proteins diff used from the discs were distributed in the plates. Identical protein patte rn (8-54 kDa molecules) of PB extract was defined by SDS-PAGE analysis in a gar strips at different distances from the discs. The predominant peptides and proteins were 8, 9, 13 and 18 kDa. The 32-36 kDa proteins were absent. These results suggest that the proteins during the maintenance on agar medi um plates were separated. Relative quantity of individual proteins was diff erent in certain distances from the discs. It is probable that these protei ns can be involved in defense of pine megagametophyte. We assume that propo rtion of individual proteins is also of importance in their defense functio n.