Purification and properties of the raw starch-degrading alpha-amylase of Bacillus sp. IMD 434

Authors
Hamilton, LM Kelly, CT Fogarty, WM
Citation
Lm. Hamilton et al., Purification and properties of the raw starch-degrading alpha-amylase of Bacillus sp. IMD 434, BIOTECH LET, 21(2), 1999, pp. 111-115
Citations number
18
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
2
Year of publication
1999
Pages
111 - 115
Database
ISI
SICI code
0141-5492(199902)21:2<111:PAPOTR>2.0.ZU;2-V
Abstract
The paw starch-degrading alpha-amylase of Bacillus sp. IMD 434 was purified to homogeneity by acetone precipitation, ion-exchange chromatography and h ydrophobic interaction chromatography. The enzyme had a relative molecular mass of 69,200, displayed maximum activity at pH 6.0 and 65 degrees C and r eleased large amounts of glucose and maltose an hydrolysis of starch.