MICROPROBE X-RAY-ABSORPTION SPECTROSCOPIC DETERMINATION OF THE OXIDATION-STATE OF INTRACELLULAR CHROMIUM FOLLOWING EXPOSURE OF V79 CHINESE-HAMSTER LUNG-CELLS TO GENOTOXIC CHROMIUM COMPLEXES

Authors
DILLON CT LAY PA CHOLEWA M LEGGE GJF BONIN AM COLLINS TJ KOSTKA KL SHEAMCCARTHY G
Citation
Ct. Dillon et al., MICROPROBE X-RAY-ABSORPTION SPECTROSCOPIC DETERMINATION OF THE OXIDATION-STATE OF INTRACELLULAR CHROMIUM FOLLOWING EXPOSURE OF V79 CHINESE-HAMSTER LUNG-CELLS TO GENOTOXIC CHROMIUM COMPLEXES, Chemical research in toxicology, 10(5), 1997, pp. 533-535
Citations number
23
Categorie Soggetti
Toxicology,Chemistry
Journal title
Chemical research in toxicology → ACNP
ISSN journal
0893228X
Volume
10
Issue
5
Year of publication
1997
Pages
533 - 535
Database
ISI
SICI code
0893-228X(1997)10:5<533:MXSDOT>2.0.ZU;2-K
Abstract
The oxidation state of intracellular chromium has been determined dire ctly in mammalian lung cells exposed to mutagenic and carcinogenic chr omium compounds. Microprobe X-ray absorption spectroscopy (XAS) experi ments on single V79 Chinese hamster lung cells showed that Cr(VI) and Cr(V) complexes were reduced completely (>90%) to Cr(III) within 4 h o f exposure of the cells. This result provides direct evidence for the hypothesis that these genotoxic oxidants react rapidly with intracellu lar reductants.