J. Szymura-oleksiak et al., Enantioselective high performance liquid chromatographic assay of acebutolol and its active metabolite diacetolol in human serum, CHIRALITY, 11(4), 1999, pp. 267-271
A stereoselective direct liquid chromatographic method for assay of the ena
ntiomers of the beta-adrenergic blocker acebutolol (AC) and its active meta
bolite, dt acetolol (DC), in human serum was developed. The assay is based
on extraction with ethyl acetate and separation of enantiomers on an amylos
e tris-(3,5-dimethylphenylcarbamate) chiral stationary phase (Chiralpak AD)
column. The method was validated and proved useful for the determination o
f the enantiomers in serum samples of patients suffering from hypertension
and chronically treated with racemic AC. The results were compared and foun
d similar with an indirect assay based on derivatization of the enantiomers
with (+)-(S)-1-(1-naphthyl)ethyl isocyanate (NEIC), Chirality 11,267-271,
1999. (C) 1999 Wiley-Liss, Inc.