CHD1 interacts with SSRP1 and depends on both its chromodomain and its ATPase/helicase-like domain for proper association with chromatin

CHD1, an M-r similar to 200,000 protein that contains a chromodomain (C), a n ATPase/helicase-like domain (H) and a DNA-binding domain (D), was previou sly shown to be associated with decompacted interphase chromatin in mammali an cells and with transcriptionally active puffs and interbands in Drosophi la polytene chromosomes. We now show by transient transfection experiments with genes expressing wild-type and mutant forms of CHD1 that both the C an d H domains are essential for its proper association with chromatin. We als o present evidence for an in vivo interaction between CHD1 and a novel HMG box-containing protein, SSRP1, which involves an amino-terminal segment of CHD1 that does not include the chromodomain. Immunocytochemical analyses in dicated that CHD1 and SSRP1 colocalize in both mammalian nuclei and Drosoph ila polytene chromosomes.