P. Brenneisen et al., Ultraviolet-B induction of interstitial collagenase and stromelyin-1 occurs in human dermal fibroblasts via an autocrine interleukin-6-dependent loop, FEBS LETTER, 449(1), 1999, pp. 36-40
Ultraviolet-B irradiation of human dermal fibroblasts has earlier been show
n to induce matrix-degrading metalloproteinases, thus driving connective ti
ssue degradation in photoaging and photocarcinogenesis. Herein,,vc report t
hat Ultraviolet-B irradiation led to a dramatic increase in specific mRNA a
nd protein levels of interstitial collagenase, stromelysin and interleukin-
6. By contrast, the major tissue inhibitor of matrix-degrading metalloprote
inases, TIMP-1, was unaffected, Monospecific neutralizing antibodies direct
ed against human interleukin-6 significantly reduced the interstitial colla
genase and stromelysin-1 protein levels, Taken together, our data provide t
he first evidence that Ultraviolet-B induction of interstitial collagenase
and stromelysin-l occurs, ia the synthesis and release of interleukin-6. He
nce, this newly identified autocrine mechanism may contribute to dermal pho
todamage. (C) 1999 Federation of European Biochemical Societies.