Yc. Lu et al., Salivary estradiol and progesterone levels in conception and nonconceptioncycles in women: evaluation of a new assay for salivary estradiol, FERT STERIL, 71(5), 1999, pp. 863-868
Objective: To determine the usefulness of salivary E-2 and progesterone for
noninvasive assessment of ovarian function.
Design: Prospective study of salivary hormone levels in women planning a pr
egnancy.
Setting: Department of Obstetrics and Gynecology at Northwestern University
Medical School in Chicago, Illinois.
Patient(s): Fourteen women aged 23-39 years with regular menstrual cycles w
ho were planning a pregnancy.
Intervention(s): None.
Main Outcome Measure(s): Salivary estradiol and progesterone concentrations
.
Result(s): The sensitivity of the E-2 assay is 2.0 pmol/L; the interassay c
oefficient of variation was 5.2% (mean value 17 pmol/L). Recovery of E-2 ad
ded to saliva was 106%. The correlation with simultaneous serum samples was
0.71. Menstrual cycle patterns contained a preovulatory depression and a m
idcycle surge. By comparison with nonconception cycles, the luteal phases o
f conception cycles had significantly elevated salivary E-2 within the firs
t 5 days after ovulation. Salivary progesterone was significantly elevated
but not until the Army Medical 10 days after ovulation.
Conclusion(s): Salivary measurements of E-2 and progesterone can be used as
noninvasive methods for assessment of ovarian function. Salivary specimens
can be collected at home and brought to the laboratory for analysis, obvia
ting the need for frequent phlebotomy. The sensitivity and precision of the
salivary E-2 assay make it comparable with assays of serum E-2 for assessi
ng changes in hormone levels. (C) 1999 by American Society for Reproductive
Medicine.