The same luteinized granulosa-luteal cells from normal and superovulated human chorionic gonadotropin (hCG)-stimulated cycles contain hCG and insulin-like growth factor binding protein-1

Objective: To determine whether hCG and IGFBP-1 appear in the same or diffe rent cells and in what sequence. Design: Retrospective analysis of laboratory data. Setting: University medical center. Patient(s): Twenty-five women undergoing IVF-ET with natural cycles and 25 women having stimulated IVF-ET. Intervention(s): Cells were obtained from dominant follicles in women with natural cycles and from the follicles from hMG- and hCG-stimulated cycles. Main Outcome Measure(s): Detection and localization of hCG and IGFBP-1 in g ranulosa-luteal cells using double immunocytochemical staining. Measurement of hCG and ICFBP-1 in follicular fluid and serum. Result(s): Three types of hCC staining were found: on the cell surface, on the cell surface and in the cytoplasm, and in the cytoplasm alone. IGFBP-1 stained diffusely in the cytoplasm and was found only in those cells that w ere luteinized and contained hCG. ICFBP-1 and hCG were colocalized in the s ame cells. There was a positive correlation between follicular fluid hCG an d IGFBP-1 levels, but only in natural IVF-ET cycles. Conclusion(s): HCG-driven luteinization is required for IGFBP-1 synthesis t o take place in granulosa cells. (C) 1999 by American Society for Reproduct ive Medicine.