Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes

The serine- and arginine-rich (SR) splicing factors play an important role in both constitutive and alternative pre-mRNA splicing, and the functions o f these splicing factors are regulated by phosphorylation. We have previous ly characterized SRPK1 (SFRSK1) and SRPK2 (SFRSK2), which are highly specif ic protein kinases for the SR family of splicing factors. Here we report th e chromosomal localization of the mouse and human genes for both kinases. S RPK1 probes detected two loci that were mapped to mouse Chromosomes 17 and X using The Jackson Laboratory interspecific backcross DNA panel, and SRPK2 probes identified a single locus on mouse Chromosome 5. Using a somatic ce ll hybrid mapping panel and by fluorescence in situ hybridization, SRPK1 an d SRPK2 were respectively mapped to human chromosomes 6p21.2-p21.3 (a regio n of conserved synteny to mouse Chromosome 17) and 7q22-q31.1 (a region of conserved synteny to mouse Chromosome 5). In addition, we also found multip le SRPK-related sequences on other human chromosomes, one of which appears to correspond to a SRPK2 pseudogene on human chromosome 8. (C) 1999 Academi c Press.