Nucleotides - Part LXI - Phthaloyl strategy: A new concept of oligonucleotide synthesis

A new alternative strategy of oligonucleotide synthesis was developed by us e of thr phthaloyl protecting group for the exocyclic amino functions of th e nucleobases (see 9 - 12). This approach combines the advantages of cheap and easily accessable monomeric building blocks (see 17 - 20), standard mac hine-aided oligonucleotide synthesis, and a fast deprotection protocol whic h is orthogonal to the cleavage procedure from the solid support. The crude oligonucleotides show high purity and require, in general, no further chro matographic purification.