Ev. Korobko et al., Resistance to tumor necrosis factor induced apoptosis in vitro correlates with high metastatic capacity of cells in vivo, IMMUNOL LET, 67(2), 1999, pp. 71-76
TNF is one of the cytokines secreted by the cells of the immune system. Our
data demonstrate that those cell lines lacking capability to form metastat
ic tumors in vivo are susceptible to TNF induced apoptosis in vitro. Howeve
r, cell lines with high metastatic potential are resistant to TNF in vitro.
Furthermore, the same cell lines were resistant to cytolytic action of oth
er cytotoxic proteins secreted by LAK cells. Our data showed that TNF resis
tance in vitro correlates with the increased level of transcription factor
NF-kappa B. This finding may provide a tool to improve current protocols of
immunotherapy and insights to how tumor cells are or are not killed by LAK
cells. (C) 1999 Elsevier Science B.V. All rights reserved.