Jh. Oosterhuis et Fa. Van Der Hoorn, Testis-specific TTF-D binds to single-stranded DNA in the c-mos and Odf1 promoters and activates Odf1, J BIOL CHEM, 274(17), 1999, pp. 11708-11712
We recently identified testis-specific nuclear factor binding sites in the
testis-specific promoters of the cmos gene and the Odf1 gene, which are 80%
identical. Here we characterize a testis-specific nuclear factor, TTF-D, w
hich is able to complex with both binding sites and stimulates Odf1 promote
r activity. TTF-D is detectable in mouse testis as early as day 11 postpart
um and contains three peptides of 22, 25, and 35 kDa in size. Surprisingly,
TTF-D binds specifically to its cognate double-stranded DNA binding site a
s well as to its single-stranded DNA binding site. Both double-stranded and
single-stranded binding site oligonucleotide DNA can specifically repress
Odf1 promoter activity. Our results suggest that TTF-D is involved in posit
ive transcription regulation of a pre-meiotic and a post-meiotic gene in th
e testis.