J. Caslavska et al., Analysis of urinary drugs of abuse by a multianalyte capillary electrophoretic immunoassay, J CHROMAT A, 838(1-2), 1999, pp. 197-211
This paper characterizes a novel multianalyte competitive binding, electrok
inetic capillary-based immunoassay for urinary methadone, opiates, benzoyle
cgonine (cocaine metabolite) and amphetamines. After incubation of 25 mu l
urine with the reactants for several minutes in the presence of an internal
standard, a small aliquot of the mixture is applied onto a fused-silica ca
pillary and the unbound fluorescein labelled drug tracers are monitored by
capillary electrophoresis with on-column laser induced fluorescence detecti
on. The multianalyte assay is shown to be rapid, simple, quantitative, capa
ble of recognizing urinary drug concentrations greater than or equal to 30
ng/ml and suitable for screening of patient urines. Data are demonstrated t
o compare well with those obtained by routine screening methods based on en
zyme multiplied immunoassay techniques and fluorescence polarization immuno
assays. The electrokinetic capillary assay has been validated via analysis
of external quality control urines and confirmation analysis of patient uri
nes using GC-MS. (C) 1999 Elsevier Science B.V. All rights reserved.