Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: Detection and analysis by PCR

Campylobacter jejuni produces a toxin called cytolethal distending toxin (C DT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt g enes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter call strains tested. Restriction analyses of the cdt PCR products showed cl ear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently , it may be possible to speciate C. jejuni and C. call isolates on the basi s of restriction patterns within their cdt genes.