An unexpected behavior of horseradish peroxidase in AOT reversed microemulsion: UV spectroscopic and stopped-flow studies

Intermediate compound II and III may be formed as a result of the reaction of horseradish peroxidase with H2O2. From the UV spectra, we can see that t he stability of the intermediate compounds is worse in reverse micelle than that in aqueous solution. With the increase of the water content, the stab ility of intermediate compounds gets better, this means that the characteri stic of the water in the water pool is near that of bulk water. The results of the stopped-flow experiments show that the formation rate constant and V-initial of intermediate compound in reverse microemulsion are bigger than that in aqueous solution. However, within the longer reaction time, the di sappearance of the intermediate compound appears early in reverse microemul sion than that in aqueous solution. These results are in agreement with the result of the UV experiments.