Highly specific detection of H2O2-dependent luminol chemiluminescence in stimulated human leukocytes using polyvinyl films

When human polymorphonuclear leukocytes (PMN) were attached to glass covers lips, cells always spread and formed reactive oxygen species prior to any e xperimental stimulation. To avoid this, a polyvinylidine chloride film was used as an inactive substance to place the cells. Cells engaged in phagocyt osis on the film exhibited a specific H2O2-mediated luminol chemiluminescen ce (LCL) at the cell-particle interface; the cells stimulated with 12-O-tet radecanoylphorbol-13-acetate became aggregated and the LCL was observed at the cell-cell contact. These results corresponded well with those obtained by an electron microscopic H2O2-demonstration method.