T cell reconstitution of BB/W rats after the initiation of insulitis precipitates the onset of diabetes

One of the diabetes susceptibility genes of the BB/W (Biobreeding/Worcester ) rat maps to the lyp locus on chromosome 4, The BB/W lyp allele is respons ible for a severe peripheral T lymphopenia, Correction of this lymphopenia by transfer of normal, histocompatible T cells prevents diabetes, providing T cell reconstitution is initiated before insulitis, We have analyzed this time-dependent regulation of the diabetogenic process by normal T cells, W e demonstrate that T cell reconstitution after the initiation of insulitis precipitates the onset of diabetes through the recruitment of donor T cells to the autoimmune process, This inability of normal T cells to regulate pr imed diabetogenic BB/W T cells and their own autoreactive potential a ere o bserved when normal T cells outnumbered pathogenic T cells by approximately 1000-fold. Analysis of donor-derived T cells recovered from BB/W rats that were reconstituted before insulitis, and hence protected from diabetes, de monstrates that early T cell reconstitution of BB/W rats does not result in a long term physical or functional depletion of islet cell-specific T cell precursors among donor cells or in the expansion of T cells that can regul ate the activation and expansion of diabetogenic T cells.