Development of an HPLC/diode-array detector method for simultaneous determination of sodium benzoate and phenolic compounds in quince jam

A simple, rapid, sensitive, reproducible, and accurate reversed-phase HPLC procedure is proposed for the determination of sodium benzoate in quince ja m samples. The sample preparation was simple, involving only extraction wit h methanol. The chromatographic separation was achieved using a reversed-ph ase column Spherisorb ODS2 (5 mu m; 25.0 x 0.46 cm). Gradient elution was c arried out using water-formic acid (19:1) (A) and methanol (B). The effluen t was monitored by a diode-array detector and chromatograms were recorded a t 280 nm. The detection limit value of the method for sodium benzoate was 0 .5 mu g/mL and the method was precise (SD=0.003; CV%=2.76; n=6). Recovery v alues of sodium benzoate from spiked quince jam samples were between 94.7 a nd 100 %. This technique can also be useful for the simultaneous definition of the phenolic profile (phenolic acids, flavonoids, and glycosides of pro cyanidin polymers) of quince jam.