Identification of an in planta-induced mRNA of Plasmodiophora brassicae

Citation
S. Ito et al., Identification of an in planta-induced mRNA of Plasmodiophora brassicae, J PHYTOPATH, 147(2), 1999, pp. 79-82
Citations number
11
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
ISSN journal
09311785 → ACNP
Volume
147
Issue
2
Year of publication
1999
Pages
79 - 82
Database
ISI
SICI code
0931-1785(199902)147:2<79:IOAIPM>2.0.ZU;2-H
Abstract
RNA fingerprinting using the arbitrarily primed polymerase chain reaction ( RAP) were performed with a host-pathogen system consisting of a Chinese cab bage cultivar and two isolates of Plasmodiophora brassicae. One of the RAP products expressed specifically in a susceptible response (clubroots) was i solated, cloned, and sequenced. Northern blot analysis confirmed that the p roduct (Y10) was expressed in clubroot tissue where P, brassicae was shown to be at a vegetative growth stage. Southern blot analysis suggested that t he Y10 sequence existed as a single-copy per haploid genome of P. brassicae . Thus, Y10 sequence appears to be a transcript from P, brassicae growing i n planta.