Comparison of restriction pattern polymorphism of Mycoplasma agalactiae and Mycoplasma bovis by pulsed field gel electrophoresis

Authors
Tola, S Idini, G Rocchigiani, AM Manunta, D Angiol, PP Rocca, S Cocco, M Leori, G
Citation
S. Tola et al., Comparison of restriction pattern polymorphism of Mycoplasma agalactiae and Mycoplasma bovis by pulsed field gel electrophoresis, J VET MED B, 46(3), 1999, pp. 199-206
Citations number
19
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARYPUBLIC HEALTH
ISSN journal
09311793 → ACNP
Volume
46
Issue
3
Year of publication
1999
Pages
199 - 206
Database
ISI
SICI code
0931-1793(199904)46:3<199:CORPPO>2.0.ZU;2-J
Abstract
Mycoplasma agalactiae and Mycoplasma bovis are closely related species in p hylogenetic terms. Pulsed Field Gel Electrophoresis (PFGE) of SmaI, EclXI, Bsi WI, MluI, BssHII, Sa/I, SboI, NruI and ApaI digested DNAs were used to analyse and to compare restriction fragment length polymorphism between M. agalactiae and M. bovis and to estimate their genome sizes. SmaI, EclXI and Bsi WI enzymes cleaved DNAs of both microrganisms. MluI, BssHII, SalI, Sho I and NruI digested only M. agalactiae DNA whereas ApaI cut only M. bovis D NA. The total DNA length was established to be 945 +/- 8.4 Kb for M. agalac tiae and 961 +/- 18.9 Kb for M bovis.