Yf. Dufrene et al., Influence of substratum surface properties on the organization of adsorbedcollagen films: In situ characterization by atomic force microscopy, LANGMUIR, 15(8), 1999, pp. 2871-2878
Atomic force microscopy (AFM) imaging and force-distance curves have been u
sed to investigate, in situ, the nanoscale organization of collagen adsorbe
d on polymer substrata covering a wide range of surface roughness and surfa
ce hydrophobicity: bisphenol A polycarbonate (PC), poly(ethylene terephthal
ate) (PET), and poly(vinylidene difluoride) (PVdF) used as such or treated
by an oxygen plasma discharge (ox). After collagen adsorption, PC and PCox
showed patterned structures under water, the size of which was influenced b
y substratum surface oxidation. These structures are attributed to aggregat
ed ends of collagen molecules. Extended rupture lengths were observed in th
e force-distance curves, suggesting that bundles of collagen molecules adhe
re to the AFM probe and are progressively torn out upon probe retraction. I
n contrast, on PET, PETox, PVdF, and PVdFox, adsorbed collagen formed a smo
oth, homogeneous film devoid of any topographic feature, and no extended ru
pture lengths were observed. After drying, holes in the collagen film were
observed on PET and PVdF and not on PETox and PVdFox. The influence of subs
tratum roughness and physicochemical properties is discussed, considering t
he mobility of collagen molecules at the interface.