In this study, lactose determination was performed comparatively by the app
lication of 2 different methods: Teles method and Biochemistry Analyzer. Ex
periments were applied in the model system and reconstituted whey system. T
he results were compared and statistically evaluated. The difference betwee
n the 2 methods was not significant. The applicability and sensitivity of t
he methods were discussed. The results indicated that the sensitivity of Te
les method and Biochemistry Analyzer depends on the optimum concentration o
f working solution. Biochemistry analyzer could be recommended for the lact
ose determination having important advantages in application.