The aim of this experiment was to evaluate the suitability of the fermentat
ion produced chymosin from E. coli (CHY-MAX rennet) in the manufacture of F
eta cheese. Five trials of paired cheesemaking were carried out using also
calf rennet as control coagulant. In general, no difference between the che
eses made with the 2 coagulants was noted throughout the cheese production.
Both rennets resulted in curds with the same level of firmness and syneret
ic properties. Yield and chemical composition of cheeses were not significa
ntly different (p<0.05) throughout ripening and regarding the sensory quali
ty, both cheeses were equally acceptable. Finally, the fermentation produce
d chymosin from E, coli was considered to be a successful alternative of ca
lf rennet used for the manufacture of Feta cheese.