Ml. Xu et al., High-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR, and AFLP markers, MOL G GENET, 261(3), 1999, pp. 574-581
Sugarcane mosaic virus (SCMV) is one of the most important virus diseases o
f maize in Europe. Genetic analysis on backcross five (BC5) progeny derived
from the cross FAP1360A (resistant) x F7 (susceptible) confirmed that at l
east two dominant genes, Scm1 and Scm2, are required for resistance to SCMV
in the progeny of this cross. With the aid of RFLP and SSR marker analyses
, Scm1 was mapped in the region of 8.7 cM - between the nucleolus organizer
region (nor) and RFLP marker bnl6.29 on the short arm of chromosome 6, whi
le Scm2 was mapped to an interval of 26.8 cM flanked by the RFLP markers um
c92 and umc102 near the centromere region of chromosome 3. Both chromosome
regions were further enriched for AFLP markers by successful application of
a bulked segregant analysis to this oligogenic trait. A total of 23 linked
AFLP markers were identified, clustered in chromosome regions adjacent to
either Scm1 or Scm2. Seven AFLP markers linked to Scm1 resided within the n
or-bnl6.29 interval, and one of them, E3M8-1, showed no recombination with
Scm1. Three AFLP markers linked to Scm2 are located between umc92 and umc10
2.