Ii. Tulokhonov et al., Interaction of isolated sigma-70 subunit of RNA polymerase with oligodeoxyribonucleotides corresponding to regions -10 and -35 of the spc promoter, MOL BIOL, 33(2), 1999, pp. 140-143
Binding of the isolated 70-kDa sigma subunit (sigma-70) of Escherichia coli
RNA polymerase with oligodeoxyribonucleotides (ON) corresponding to region
s -10 and -35 of the spc promoter of E. coli ribosomal genes was studied by
gel filtration and gel retardation assay. The preparation of sigma-70 was
shown to contain no admixtures of DNA-binding proteins using 4-[N-methyl-N-
(2-chloroethyl)amino]benzyl-5'-phosphamide derivatives of ON.