Cycloprodigiosin uncouples H+-pyrophosphatase of plant vacuolar membranes in the presence of chloride ion

Citation
M. Maeshima et al., Cycloprodigiosin uncouples H+-pyrophosphatase of plant vacuolar membranes in the presence of chloride ion, PLANT CEL P, 40(4), 1999, pp. 439-442
Citations number
17
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
00320781 → ACNP
Volume
40
Issue
4
Year of publication
1999
Pages
439 - 442
Database
ISI
SICI code
0032-0781(199904)40:4<439:CUHOPV>2.0.ZU;2-8
Abstract
Cycloprodigiosin hydrochloride (cPrG-HCl) at 10 nM uncoupled both H+-ATPase and H+-translocating pyrophosphatase in vacuolar membrane vesicles of mung bean, but did not inhibit the substrate hydrolysis. cPrG-HCl destroyed the transmembrane pH gradient only in the presence of Cl-. The observations in dicate that cPrG-HCl functions as a H+/Cl- symporter. Vesicle acidification was restored by SO42- even in the presence of cPrG-HCl.