In vitro antifungal susceptibilities of Candida albicans and other fungal pathogens to polygodial, a sesquiterpene dialdehyde

In vitro antifungal activities of polygodial were investigated against seve ral fungal pathogens. Polygodial showed strong antifungal activity, compara ble to amphotericin B, against yeast-like fungi Candida albicans, C. utilis , C. krusei. Cryptococcus neoformans. Saccharomyces cerevisiae and also fil amentous fungi including Trichophyton mentagrophytes, T. ruburum. and Penic illium marneffei. Other strains such as Aspergillus fumigatus, A. flavus, P . chrysogenum, C. lipolytica, and C. tropicalis showed moderate to low susc eptibility to polygodial. The antifungal activity of polygodial was general ly not reduced by several susceptibility-testing conditions such as medium, incubation temperature, inoculum size, and medium pH. However, polygodial' s antifungal activity was strongly increased at acidic conditions. Unlike a mphotericin B, polygodial did not show any hemolytic activity and also its antifungal activity was not diminished in the presence of ergosterol. Based on killing kinetics against growing and nongrowing C. albicans, polygodial showed strong and rapid fungicidal activity.