Splenic marginal metallophilic macrophages and marginal zone macrophages are the major interferon-alpha/beta producers in mice upon intravenous challenge with herpes simplex virus
Ml. Eloranta et Gv. Alm, Splenic marginal metallophilic macrophages and marginal zone macrophages are the major interferon-alpha/beta producers in mice upon intravenous challenge with herpes simplex virus, SC J IMMUN, 49(4), 1999, pp. 391-394
The interferon (IFN)-alpha/beta-producing cells (IPCs) are localized predom
inantly in the spleen, in particular in the marginal zones (MZ), in C57BL/6
mice injected intravenously (i.v.) with UV-inactivated herpes simplex viru
s (HSV). We defined the phenotype of these murine IPCs using simultaneous i
mmunohistochemical labelling of intracellular IFN-alpha/beta and various su
rface antigens. We found that the IPCs in the MZ are not dendritic cells be
cause they did not express major histocompatibility complex (MHC) class II
and CD11c molecules. Furthermore, they did not express antigenic markers ty
pical for Tcells, B cells or red pulp macrophages. In contrast, the majorit
y of IPCs were stained by the anti-sialoadhesin monoclonal antibody (MoAb)
SER-4, which is specific for marginal metallophilic macrophages. In additio
n, a minor part of the IPCs with a more outward localization in the MZ were
stained by a MoAb specific for MZ macrophages. We conclude that the massiv
e IFN-alpha/beta production in the MZ of the spleen upon in vivo, stimulati
on by HSV is mainly exerted by marginal metallophilic macrophages and to a
lesser extent by MZ macrophages.