DISPLACEMENT ELECTROPHORESIS OF AMPHOLYTES IN A CONTINUOUS PH GRADIENT MOVING IN A CAPILLARY WITH A NONCONSTANT CROSS-SECTION

Our previous contribution described the construction and function of m iniaturized automated instrumentation for displacement electrophoresis using a separation compartment consisting of a tapered channel couple d to a constant cross-section detection fused-silica capillary. in thi s work, the above instrumentation is used to study the separation and focusing of the zones of ampholytes during their movement on the backg round of continuous pH gradient generated by carrier ampholytes. Depen dencies of the resolution of chosen colored low-molecular-mass ampholy tes on the working parameters - including the length of the detection fused-silica capillary, concentration of counterion in the leading ele ctrolyte, electric current, amount of carrier ampholytes and the analy te acidobasic properties - are evaluated. A reasonable speed of separa tion and resolution can be achieved at a potential drop over the separ ation compartment down to 200 V.