A study on the reaction mechanism between chrome-azurol S and bovine serumalbumin

Citation
Cq. Ma et al., A study on the reaction mechanism between chrome-azurol S and bovine serumalbumin, ACT CHIM S, 57(4), 1999, pp. 389-395
Citations number
9
Categorie Soggetti
Chemistry
Journal title
ACTA CHIMICA SINICA
ISSN journal
05677351 → ACNP
Volume
57
Issue
4
Year of publication
1999
Pages
389 - 395
Database
ISI
SICI code
0567-7351(1999)57:4<389:ASOTRM>2.0.ZU;2-3
Abstract
In this paper, three different methods were used to study the binding numbe rs and binding constants of chrome-azurol S (CAS) on bovine serum albumin ( BSA), and almost same results were obtained. It is proposed that the concen tration of CAS and pH of the solutions affect the binding mode of CAS and B SA. According to the theory of Forster non-radiative energy transfer, the b inding sites of CAS on BSA are found. Taking advantage of fluorescence quen ching, the reaction mechanism of CAS and BSA is explored.