S. Aleksic-kovacevic et al., Immunohistochemical detection of infectious bursal disease virus antigen in the bursa of fabricius of experimentally infected chickens, ACT VET BEO, 49(1), 1999, pp. 13-19
The indirect immunoperoxidase method (IP) with polyclonal antibodies was us
ed for detecting infectious bursal disease (IBD) virus antigen in the bursa
of chickens experimentally infected with a highly virulent vi,vs designate
d as KA. Detection of IBD virus by the IP method on methanol fixed paraffin
embedded tissue samples is possible ten days after infection (PI). Three t
o seven days PI an intensive expression of IBDV antigen can be observed on
lymphocytes in the cortex and medulla of bursal follicles, as well as on ma
crophages in the follicles and interstitium. Ten days PI the immunohistoche
mical reaction is very discreet, but IBD virus detection is still possible.
The IP method, used in our work, is a sensitive and specific way of demons
trating IBDV in the bursa of Fabricius of experimentally infected chickens.