Grain dust induces IL-8 production from bronchial epithelial cells: the effect of dexamethasone on IL-8 production

Background: Recent publications have suggested an active participation of n eutrophils to induce bronchoconstriction after inhalation of grain dust (GD ). Objective: To further understand the role of neutrophils in the pathogen esis of GD-induced asthma, this investigation was designed to determine whe ther human bronchial epithelial cells could produce IL-8 production and to observe the effect of dexamethasone on IL-8 production. Materials and methods: We cultured Beas-2B, a bronchial epithelial cell lin e. To observe GD-induced responses, four concentrations (1 to 200 mu g/mL) of GD were incubated for 24 hours and compared with those without incubatio n of GD. To evaluate the effect of pro-inflammatory cytokines on IL-8 produ ction, epithelial cells were incubated with peripheral blood mononuclear ce ll (PBMC) culture supernatant, which was derived from the culture of PBMC f rom a GD-induced asthmatic subject under the exposure to 10 mu g/mL of GD, and compared with those cultured without addition of PBMC supernatant. The level of released IL-8 in the supernatant was measured by enzyme-linked imm unosorbent assay. To evaluate the effect of dexamethasone an IL-8 productio n, four concentrations (5 to 5000 ng/mL) of dexamethasone were pre-incubate d for 24 hours and the same experiments were repeated. Results: There was significant production of IL-8 from bronchial epithelial cells with additions of GD in a dose-dependent manner (P < .05), which was significantly augmented with additions of PBMC supernatant (P < .05) at ea ch concentration. Compared with the untreated sample, pretreatment of dexam ethasone could induced a remarkable inhibitions (15% to 55%) of IL-8 produc tion from bronchial epithelial cells in a dose-dependent manner. Conclusion: These results suggest that IL-8 production from bronchial epith elial cells may contribute to neutrophil recruitment occurring in GD-induce d airway inflammation. The downregulation of IL-8 production by dexamethaso ne from branchial epithelial cells may contribute to the efficacy of this c ompound in reducing cellular infiltration and ultimately to its anti-inflam matory property.