Detection of bacteriocins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Authors
Rose, NL Sporns, P McMullen, LM
Citation
Nl. Rose et al., Detection of bacteriocins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, APPL ENVIR, 65(5), 1999, pp. 2238-2242
Citations number
27
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
5
Year of publication
1999
Pages
2238 - 2242
Database
ISI
SICI code
0099-2240(199905)65:5<2238:DOBBML>2.0.ZU;2-K
Abstract
The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the detection of bacteriocins was investiga ted. A 30-s water wash of the sample on the MALDI-TOF MS probe was effectiv e in removing contaminants of the analyte. This method was used for rapid d etection of nisin, pediocin, brochocin A and B, and enterocin A and B from culture supernatants and For detection of enterocin B throughout its purifi cation.