Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR

Authors
Burtscher, C Fall, PA Wilderer, FA Wuertz, S
Citation
C. Burtscher et al., Detection of Salmonella spp. and Listeria monocytogenes in suspended organic waste by nucleic acid extraction and PCR, APPL ENVIR, 65(5), 1999, pp. 2235-2237
Citations number
9
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
5
Year of publication
1999
Pages
2235 - 2237
Database
ISI
SICI code
0099-2240(199905)65:5<2235:DOSSAL>2.0.ZU;2-4
Abstract
A nucleic acid-based method for the detection of the bacterial pathogens Sa lmonella spp. and Listeria monocytogenes in biological waste was developed. The detection limits were less than 10 cells per mi of biological waste. T he method does not include a phenol extraction step and can be easily perfo rmed in 1 to 2 days.