Studies on alkaline protease produced by Bacillus sp NG312

Authors
S, J Gill, N Devasahayam, G Sahoo, DK
Citation
J. S et al., Studies on alkaline protease produced by Bacillus sp NG312, APPL BIOC B, 76(1), 1999, pp. 57-63
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology","Biochemistry & Biophysics
Journal title
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN journal
02732289 → ACNP
Volume
76
Issue
1
Year of publication
1999
Pages
57 - 63
Database
ISI
SICI code
0273-2289(199901)76:1<57:SOAPPB>2.0.ZU;2-M
Abstract
An alkalophilic hyperproducer of alkaline protease, Bacillus sp. NG312, was isolated, and the enzyme showed maximum activity at PH 11.0 and 60 degrees C. The temperature optimum was increased by 10 degrees C in presence of Ca 2+. The crude enzyme was found to have half-life of 11 d at 37 degrees C an d maximum stability at pH 9.0-10.0. It also exhibited very good stability i n presence of detergent components and some locally available commercial de tergent powders.