Detection of equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) in Przewalski's wild horses

Authors
Borchers, K Frolich, K Ludwig, H
Citation
K. Borchers et al., Detection of equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) in Przewalski's wild horses, ARCH VIROL, 144(4), 1999, pp. 771-780
Citations number
25
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
144
Issue
4
Year of publication
1999
Pages
771 - 780
Database
ISI
SICI code
0304-8608(1999)144:4<771:DOEHT2>2.0.ZU;2-N
Abstract
In blood samples of seven captive equid species from four German toes EHV-1 specific antibodies were detected in 76% and EHV-4 specific antibodies in 73% of the 55 animals, whereas 93% were tested positive for EHV-2 and EHV-5 , respectively. In only one blood sample from a Przewalski's wild horse EHV -4 DNA was amplified by PCR. From seven Przewalski's wild horses EHV-2, and from another one EHV-5 was isolated by cocultivation. The identity of the virus isolates was verified by PCR and restriction enzyme digestion.