T. Toyoshima et al., Nitric oxide up-regulates the expression of intercellular adhesion molecule-1 on cancer cells, BIOC BIOP R, 257(2), 1999, pp. 395-399
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Nitric oxide (NO) is an unstable free radical that functions as a cytotoxic
agent secreted by macrophages to kill cancer cells. Here we report the eff
ect of NO on the expression of intercellular adhesion molecule-1 (ICAM-1) o
n cancer cells. NO donors such as SNP, SNAP and SIN-1 up-regulated the expr
ession of ICAM-1 on NA cells, a squamous cell carcinoma cell line. Northern
blot analysis showed that the induction of ICAM-1 might be due to transcri
ptional induction of ICAM-1 mRNA. Up-regulation of ICAM-1 mBNA by NO donors
was inhibited by carboxy-PTIO, a NO scavenger. Although NF-kappa B activit
y was induced by NO donors, AP-1 was not induced by them. Staurosporin, a p
rotein kinase C (PKC) inhibitor, inhibited the induction of ICAM-1 on NA ce
lls by NO, whereas genistein, a protein tyrosine kinase inhibitor, did not.
These findings indicate that NO up-regulates ICAM-1 expression on cancer c
ells by a regulatory mechanism involving PKC and suggest that NF-kappa B, b
ut not AP-1, might be involved in induction of ICAM-1 by NO in cancer cells
. (C) 1999 Academic Press.