Ik. Sharma et Dl. Jones, Characterisation of natural hybrids between Pterostylis alveata Garnet andPterostylis ophioglossa R. Br. (Orchidaceae) by starch gel electrophoresis, BIOCH SYST, 27(5), 1999, pp. 499-505
A putative natural inter-specific orchid hybrid between Pterostylis alveata
and P. ophioglossa from Fingal Point, New South Wales, Australia, with int
ermediate morphological characters between the parents was tested using sta
rch gel electrophoresis. Four enzyme systems, glucose phosphate isomerase (
GPI), uridine diphosphogluconic pyrophosphatase (UDP), malic enzyme (ME) an
d leucine amino-peptidase (LAP), exhibited precisely the heterozygote patte
rn in the hybrid plants demonstrating the presence of alleles from both par
ents and distinguishing hybrid genotypes from the parental genotype. (C) 19
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