Site-specific characterization of the N-linked glycans of murine prion protein by high-performance liquid chromatography electrospray mass spectrometry and exoglycosidase digestions

The murine prion protein PrP gene encodes a protein of 254 amino acids with two consensus sites for Asn-linked glycosylation at codons 180 and 196. A partial site-specific study of the N-linked glycans from hamster PrP has pr eviously been carried out by mass spectrometry [Stahl, N., Baldwin, M. A., Teplow, D. B., Hood, L., Gibson, B. W., Burlingame, A. L., and Prusiner, S. B. (1993) Biochemistry 32, 1991-2002] and revealed that the glycosylation at Asn-181 (equivalent to mouse 180) is heterogeneous, comprising over 30 g lycoforms. The identification of the glycosylated peptide spanning Asn-197 was not reported. Recent technical advances in electrospray mass spectromet ry now provide the sensitivity to detect low femtomole quantities of glycop eptides with >5000 mass resolution and 30 ppm mass measurement [Medzihradsz ky, K. F., Besman, M. J., and Burlingame, A. L. (1998) Rapid Commun. Mass S pectrom. 12, 472-478]. This performance coupled with stepwise exoglycosidas e digestion has been employed to establish the differential nature of the s tructural complexity (glycoforms) of the glycans at Asn-180 and Asn-196 fro m a single strain infected with the ME7 strain. Some sixty structures have been found characterized by neutral and sialylated bi-, tri-, and tetraante nnary complex-type bearing outer-arm alpha(1-3)-fucosylation (the Lewis(x) and sialyl-Lewis(x) epitopes), core alpha(1,6) fucosylation, and the presen ce of terminal HexNAc residues. The Lewis(x) trisaccharide is the major non reducing structure at Asn-180, and significant amounts of both Lewis(x) and sialyl Lewis(x) epitopes are observed at Asn-196. The abundance of the Lew is(x) and sialyl Lewis(x) epitopes on murine PrPSc may indicate a role for these structures in the normal function of PrPC or the pathophysiology of P rPSc.