Ja. Kornblatt et al., The individual tyrosines of proteins: their spectra may or may not differ from those in water or other solvents, BBA-PROT ST, 1431(1), 1999, pp. 238-248
Citations number
48
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
The overall derivative spectrum of a protein is the sum of the individual d
erivative spectra just as the overall ultraviolet spectrum of a protein is
the sum of its component parts, The RNase and DNA binding protein Sso7d has
two tyrosines and one tryptophan. We used two mutant forms of the protein
to show that the individual aromatics contribute derivative spectra that ca
n be explained on the basis of their environments. We used mutant forms of
iso-l-cytochrome c to estimate the contributions of the single tryptophan a
nd three of the five tyrosines to the overall derivative spectrum. The tryp
tophan spectrum is not exceptional. The comparable tyrosine spectra are mor
e complex. The derivative spectrum of individual tyrosines does not corresp
ond to that expected on the basis of concentration. This is a reflection of
two factors: (1) the extent to which mutations are sensed distally through
the introduction and compression of packing defects; and (2) the extent to
which electronic transitions of tyrosine are influenced by nearby atoms. T
his influence could take the form of tyrosine residing in an area where the
dielectric coefficient is not uniform; it could also result from tyrosine
bumping into neighboring atoms with lower frequency than it does in solutio
n, (C) 1999 Elsevier Science B.V, All rights reserved.