Synthesis of a new fluorogenic substrate for the continuous assay of mammalian phosphoinositide-specific phospholipase C

Authors
Rukavishnikov, AV Zaikova, TO Birrell, GB Keana, JFW Griffith, OH
Citation
Av. Rukavishnikov et al., Synthesis of a new fluorogenic substrate for the continuous assay of mammalian phosphoinositide-specific phospholipase C, BIOORG MED, 9(8), 1999, pp. 1133-1136
Citations number
7
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
8
Year of publication
1999
Pages
1133 - 1136
Database
ISI
SICI code
0960-894X(19990419)9:8<1133:SOANFS>2.0.ZU;2-J
Abstract
The synthesis of a fluorogenic substrate for mammalian phosphoinositide-spe cific phospholipase C is described. The substrate, based on the widely used fluorescein molecule? is a water-soluble substrate analog of phosphatidyli nositol-4-phosphate. The fluorogenic substrate 2 is shown to be a sensitive substrate for human PI-PLC-delta 1 in a continuous assay. (C) 1999 Elsevie r Science Ltd. All rights reserved.