Double staining of skeleton using microwave irradiation

Authors
Ilgaz, C Kocabiyik, H Erdogan, D Ozogul, C Peker, T
Citation
C. Ilgaz et al., Double staining of skeleton using microwave irradiation, BIOTECH HIS, 74(2), 1999, pp. 57-63
Citations number
34
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
BIOTECHNIC & HISTOCHEMISTRY
ISSN journal
10520295 → ACNP
Volume
74
Issue
2
Year of publication
1999
Pages
57 - 63
Database
ISI
SICI code
1052-0295(199903)74:2<57:DSOSUM>2.0.ZU;2-L
Abstract
The fetal skeleton double staining method is used to reveal developmental a bnormalities in the skeletal system. We used alizarin red S and alcian blue successfully with microwave irradiation for skeletal double staining. The fixation time was reduced from 4-7 days to 2-2.5 min and the staining time was reduced from 4 days to 23 min.