Authors
Citation
L. Marcinkeviciene et al., Purification and characterisation of PQQ-dependent glucose dehydrogenase from Erwinia sp. 34-1, BIOTECH LET, 21(3), 1999, pp. 187-192
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492
→ ACNP
Volume
21
Issue
3
Year of publication
1999
Pages
187 - 192
Database
ISI
SICI code
0141-5492(199903)21:3<187:PACOPG>2.0.ZU;2-D
Abstract
A pyrroloquinoline quinone-dependent glucose dehydrogenase from an isolate
of Erwinia sp. has been purified to homogeneity and characterised. SDS-PAGE
showed a single band of 88.4 kDa. The enzyme activity was optimal at 47 de
grees C and pH 7.5-8.5. The Michaelis constants for D-glucose and PMS were
3.2 mM and 132 mu M, respectively (50 mM glycine-NaOH, at pH 8.0).