Production of poly(4-hydroxybutyric acid) by fed-batch cultures of recombinant strains of Escherichia coli

Authors
Song, SS Hein, S Steinbuchel, A
Citation
Ss. Song et al., Production of poly(4-hydroxybutyric acid) by fed-batch cultures of recombinant strains of Escherichia coli, BIOTECH LET, 21(3), 1999, pp. 193-197
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
3
Year of publication
1999
Pages
193 - 197
Database
ISI
SICI code
0141-5492(199903)21:3<193:POPABF>2.0.ZU;2-I
Abstract
A recombinant strain of Escherichia coli was used to produce poly(4-hydroxy butyric acid), P(4HB), homopolyester by fed-batch culture in M9 mineral sal ts medium containing glucose and 4-hydroxybutyric acid as carbon sources. T he final cell dry weight, P(4HB) concentration and P( I HB) content were 12 .6 g/l, 4.4 g/l, and 36% of cell dry weight, respectively, in a 27-1 stirre d and aerated fermenter after 60 h of fed-batch fermentation at constant pH .