Evaluation of different promoters for the efficient production of heterologous proteins in baker's yeast

Authors
Monfort, A Finger, S Sanz, P Prieto, JA
Citation
A. Monfort et al., Evaluation of different promoters for the efficient production of heterologous proteins in baker's yeast, BIOTECH LET, 21(3), 1999, pp. 225-229
Citations number
16
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
21
Issue
3
Year of publication
1999
Pages
225 - 229
Database
ISI
SICI code
0141-5492(199903)21:3<225:EODPFT>2.0.ZU;2-5
Abstract
We have constructed reporter gene expression vectors placing the Aspergillu s oryzae alpha-amylase cDNA and the Geotrichum candidum lipase 2 gene, unde r the control of the yeast gene promoters,ACT1, ADH1, PGK1, and TDH1. Expre ssion regulated by the shorted form of the ADH1 promoter gave the highest p roteins yield for cells cultured in molasses medium or flour/water mixtures . Nevertheless, the ACT1 promoter appeared as the strongest in cells growin g actively with sucrose.