Evaluation of trichloroethylene degradation by E-coli transformed with dimethyl sulfide monooxygenase genes and/or cumene dioxygenase genes

Pseudomonas fluorescens IP01 grown on isopropylbenzene (cumene) and Acineto bacter sp. 20B grown on dimethyl sulfide (DMS) degraded up to 90% and 25% o f 1.5 mg trichloroethylene (TCE)/l, respectively. Escherichia coli harborin g the DMS monooxygenase genes from strain 20B, the cumene dioxygenase genes from strain IP01 and both oxygenase genes, degraded up to 50%, 75% and 88% of 75 mg TCE/l, respectively. The growth rates of the E. coli recombinants remained nearly unaffected by TCE at 15 150 mg/l. Thus, the E. coli recomb inants were indicated to degrade high concentrations of TCE efficiently at least up to 150 mg l(-1).