Pulsed column reactor as a novel tool for continuous enzymatic synthesis of peptide in an aqueous/organic biphasic medium

We propose a novel effective method for a continuous peptide synthesis in a n aqueous/organic biphasic medium using a pulsed column reactor. N-Formyl-L -aspartyl-L-phenylalanine methyl ester was enzymatically synthesized contin uously. With this extractive method using a pulsed column reactor, we can s ynthesize peptides with a stable performance even if a peptide (or a peptid e-amino acid complex) is precipitated due to its high hydrophobicity.