Synthesis of new oligosaccharides from raffinose by Aspergillus niger fructosyltransferase

Purified fructosyltransferase from Aspergillus niger exhibited transfructos ylation activities, producing fructose, DP2, DP4, and DP5 from raffinose. T he structures of two products synthesized from raffinose were identified as O-alpha-D-galactopyranosyl (1 --> 6)-alpha-D-glucopyranose and O-alpha-D-g alactopyranosyl (1 --> 6)-alpha-D-glucopyranosyl [O-beta-D-fructofuranosyl (2 --> 1)]-beta-D-fructofuranoside, which means that C-2 hydroxyl group of fructose released from one raffinose molecule were linked to the C-1 hydrox yl group of fructose of another raffinose.