Optimisation of a cyanobacterial culture used for production of isotopes-labelled recombinant proteins

Citation
G. Brandner et al., Optimisation of a cyanobacterial culture used for production of isotopes-labelled recombinant proteins, BIOTECH TEC, 13(3), 1999, pp. 177-180
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOTECHNOLOGY TECHNIQUES
ISSN journal
0951208X → ACNP
Volume
13
Issue
3
Year of publication
1999
Pages
177 - 180
Database
ISI
SICI code
0951-208X(199903)13:3<177:OOACCU>2.0.ZU;2-J
Abstract
A culture of Spirulina platensis was optimised for the preparation of an is otopes-labelled medium that can be used for cultivation of E. coli. Optimis ed conditions include 0.625 g [N-13]-NaNO3/l and 5.6 g [C-13]-NaHCO3/l, and maintenance of a basic pH. The medium produced from the hydrolysed cyanoba cterial biomass supported the growth of E. coli with a doubling time and bi omass comparable to those obtained with the rich medium LB. This procedure allowed a reduction of the costs of isotope labelling of recombinant protei ns by a factor of about 3.